Cloning of full-length cDNA of teleost corticotropin-releasing hormone precursor by improved inverse PCR.
نویسندگان
چکیده
We designed a new inverse PCR protocol combined with switching mechanism at 5' end of RNA transcript (SMART) technology, and applied it to the cloning of teleost corticotropin-releasing hormone precursor cDNA. Due to the advantages of both techniques, this method can efficiently amplify the complete 5'- and 3'-ends of cDNA in a single reaction, and might prove to be an alternative to the conventional rapid amplification of cDNA ends (RACE) approaches.
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عنوان ژورنال:
- Bioscience, biotechnology, and biochemistry
دوره 70 8 شماره
صفحات -
تاریخ انتشار 2006